Adipose tissue-derived lipid metabolites mediate fasting- induced anti-inflammatory effect on liver
- 주제(키워드) 단식 , 염증반응 , 지방간 , 지질 대사체 , 기관 간 상호작용 , fasting , inflammation , MASLD , lipid metabolite , interorgan crosstalk
- 발행기관 서강대학교 일반대학원
- 지도교수 허진영
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제URI http://www.dcollection.net/handler/sogang/000000082768
- UCI I804:11029-000000082768
- 본문언어 영어
- 저작권 논문은 저작권에 의해 보호받습니다.
목차
I. Introduction 1
(1) Metabolic dysfunction associated steatotic liver disease 1
(2) Adipose tissue-liver crosstalk and metabolic homeostasis 1
(3) The anti-inflammatory effect of fasting on liver 2
(4) Adipose tissue as an endocrine organ 3
(5) Research Objective 4
II. Materials & Methods 6
(1) Animals 6
(2) Primary hepatocyte isolation 6
(3) Co-culture of primary hepatocyte and adipose tissue 8
(4) Ex vivo lipolysis and Conditioned media(CM) preparation 8
(5) Lipid extraction 9
(6) Thin layer chromatography and lipid recovery 9
(7) Western blot 10
(8) qRT-PCR 11
(9) NEFA(non-esterified fatty acid) assay 11
(10) Lipidomics analysis 12
(11) Statistical analysis 12
III. Results 16
(1) Fasting exerts anti-inflammatory effect on liver 16
(2) Co-culture with fasted eWAT and conditioned media treatment changes inflammation level 21
(3) Lipids induced by ex vivo lipolysis can alter inflammatory gene expression of mouse primary hepatocyte 26
(4) Lipidomics revealed significant difference between CL(-) and CL(+) CM 36
(5) Peroxisomal lipids were selected by public data analysis 42
(6) Searching candidates other than peroxisomal lipids 59
IV. Discussion 74
V. Conclusion 79
References 80
