Development of a Polydopamine-Silica Enzyme Immobilization Platform for CO to Formate Conversion
- 발행기관 서강대학교 일반대학원
- 지도교수 이진원
- 발행년도 2025
- 학위수여년월 2025. 8
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000082111
- UCI I804:11029-000000082111
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
초록 (요약문)
The catalytic conversion of carbon monoxide (CO) into formate under mild aqueous conditions presents a sustainable strategy for carbon valorization. In this study, we developed a polydopamine-coated silica (PDA–silica) platform for the covalent immobilization of carbon monoxide dehydrogenase (CODH) and formate dehydrogenase (FDH) thereby establishing a biocatalytic system for CO to formate conversion. The PDA–silica carriers were fabricated via dopamine self-polymerization and characterized using SEM-EDS and FTIR to confirm surface functionalization and enzyme immobilization. Immobilization parameters were systematically evaluated, and the PDA–silica system exhibited 5.9–7.3-fold higher total activity compared to conventional Ni–NTA immobilization. The immobilized enzymes retained over 60% of their initial activity after 15 days of storage and achieved high formate titers (up to 387 mM) under both batch and stirred-tank reactor (STR) conditions. Additionally, more than 46% of catalytic productivity was maintained after 10 reuse cycles, indicating robust operational stability. These findings establish the PDA–silica platform as a robust and scalable immobilization strategy, offering a practical solution for biocatalytic CO and CO₂ utilization under environmentally benign conditions.
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Contents
Abstract
1. Introduction 1
2. Material and Methods 8
2.1. Materials 8
2.2. Preparation of Polydopamine-Coated Silica Particles 8
2.3. Enzyme Preparation and Immobilization on PDA-Silica 9
2.4. Enzyme Activity Assay 10
2.5. Stability Test 12
2.6. CO to Formate Bioconversion Reaction 12
2.7. Reusability Test 13
2.8. Analytical Methods 14
2.8.1. Surface Characterization of Immobilized Carriers 14
2.8.2. Formate Analysis 15
3. Results and Discussion 16
3.1. Construction and Characterization of PDA–Silica Immobilization System 16
3.2. Evaluation of Enzyme Immobilization Efficiency and Activity 23
3.2.1. Effect of Enzyme Loading Amount 23
3.2.2. Effect of Immobilization Temperature 26
3.2.3. Immobilized Enzyme Comparison 29
3.3. Storage Stability of Immobilized Enzymes 31
3.4. CO to Formate Conversion Performance 34
3.5. Reusability of PDA-silica Immobilized Enzyme 36
4. Conclusion 39
5. Acknowledgement 41
6. References 42
