서강대학교

목차

Contents
Abstract
1. Introduction 1
2. Material and Methods 8
2.1. Materials 8
2.2. Preparation of Polydopamine-Coated Silica Particles 8
2.3. Enzyme Preparation and Immobilization on PDA-Silica 9
2.4. Enzyme Activity Assay 10
2.5. Stability Test 12
2.6. CO to Formate Bioconversion Reaction 12
2.7. Reusability Test 13
2.8. Analytical Methods 14
2.8.1. Surface Characterization of Immobilized Carriers 14
2.8.2. Formate Analysis 15
3. Results and Discussion 16
3.1. Construction and Characterization of PDA–Silica Immobilization System 16
3.2. Evaluation of Enzyme Immobilization Efficiency and Activity 23
3.2.1. Effect of Enzyme Loading Amount 23
3.2.2. Effect of Immobilization Temperature 26
3.2.3. Immobilized Enzyme Comparison 29
3.3. Storage Stability of Immobilized Enzymes 31
3.4. CO to Formate Conversion Performance 34
3.5. Reusability of PDA-silica Immobilized Enzyme 36
4. Conclusion 39
5. Acknowledgement 41
6. References 42