Selective Interaction of Imidazolium-based Amphiphile Molecules with Differently Charged Liposomes
- 주제어 (키워드) GUV , selective permeability , IMS , Bulky-IMS , Methoxy-IMS , controlled pore-formation
- 발행기관 서강대학교 일반대학원
- 지도교수 신 관 우
- 발행년도 2025
- 학위수여년월 2025. 8
- 학위명 석사
- 학과 및 전공 일반대학원 화학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000081795
- UCI I804:11029-000000081795
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
초록 (요약문)
Imidazolium-based amphiphiles (IMS) are emerging as powerful tools for modulating membrane permeability in synthetic lipid systems. In this study, we investigate and compare two IMS derivatives Bulky-IMS, with branched hydrophobic moieties, and Methoxy-IMS, with smaller, less hydrophobic substitutions for their effects on membrane structure and stability. Using dye-loaded giant unilamellar vesicles (GUVs) composed of zwitterionic DOPC and anionic DOPG lipids, we show that Bulky-IMS induces significant membrane permeabilization, particularly in DOPG vesicles, while Methoxy-IMS demonstrates minimal disruptive activity. Confocal fluorescence imaging and 3D membrane morphology reveal permeability changes. Sequential and combinatorial treatments (one-dish experiments), along with osmotic shock experiments, further support the role of amphiphile hydrophobicity and membrane charge in vesicle permeability. These findings highlight the potential of IMS compounds for selective and tunable control of membrane permeability in synthetic cell models.
more목차
1. Introduction 1
2. Experimental Methods 10
2.1 Materials 10
3. Results 13
3.1 Effect of Bulky-IMS on Vesicles’ Permeability 13
3.2 Effect of Methoxy-IMS on Vesicles’ Permeability 17
3.3 Direct Comparison of IMS 19
3.4 Control Experiments 23
4. Discussion 25
5. Conclusion 27
References 29
