Enhanced Sonodynamic Therapy using Focused Ultrasound-Activated Oxygen Carrying Nanodroplet for the Treatment of Hypoxic Tumors
- 주제(키워드) Nanodroplet , Focused Ultrasound , Sonosensitizer , Hypoxia , Oxygen , Sonodynamic therapy
- 발행기관 서강대학교 일반대학원
- 지도교수 김현철
- 발행년도 2021
- 학위수여년월 2021. 8
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- UCI I804:11029-000000066029
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권보호를 받습니다.
초록/요약
Recently, sonodynamic therapy using sonosensitizers encapsulating nanoparticles has been intensively investigated, due to the limitation of photodynamic therapy of the light penetration depth. However, the hypoxic condition of cancer tissue limits the effectiveness of sonodynamic therapy. In this study, we loaded decafluoropentane (DFP) with high oxygen carrying capability in the core of a phospholipid based nanodroplet. Also, for sonodynamic therapy, verteporfin was loaded in the nanodroplet. When the nanodroplets are delivered to the cancer tissue, focused ultrasound (FUS) is irradiated for the evaporation of the DFP and the cavitation of nanodroplets, while temporarily generating heat and releasing excess oxygen to overcome hypoxic condition. The FUS-activated verteporfin loaded nanodroplet generated sufficient amounts of ROS and demonstrated improved sonodynamic therapy efficacy in in vitro and in vivo experiments. In conclusion, the DFP and verteporfin loading nanodroplet showed sufficient potential in cancer therapy by overcoming the hypoxia condition of tumor environment and the limitations of PDT.
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