Optimization of fermentation condition for 3-hydroxypropionic acid production from methane by metabolically engineered Methylosinus trichosporium OB3b
- 주제(키워드) 3-hydroxypropionic acid , Biooreactor cultivation , Methylosinus trichosporium OB3b , Methane
- 발행기관 서강대학교 일반대학원
- 지도교수 나정걸
- 발행년도 2021
- 학위수여년월 2021. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- UCI I804:11029-000000065949
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권보호를 받습니다.
초록/요약
Our growing concerns on acute environmental problems caused by reckless fossil resource usage have drawn significant attention to the bio-based production of chemicals and materials. 3-hydroxypropionic acid (3HP) is one of the top building block chemicals serving as a versatile precursor for further synthesis of various value-added chemicals such as 1,3-propanediol and acrylic acid and as a monomer for various polymers. For the efficient production of 3HP from methane, a significant greenhouse gas, a series of cultivations using recombinant M. trichosporium OB3b, a type II methanotroph, have been performed based on the metabolism of the cells. To enhance the production of 3HP, carbon dioxide along with methane were supplied to support carbonate formation, which is required for conversion of acetyl-CoA into malonyl-CoA, a precursor of 3HP. Through efficient gas transfer with carbon dioxide supplement, 60.59 mg/L of 3HP with 12.83 mg/g DCW/day of productivity could be achieved after 42 h of fermentation, which was 1.24-fold and 2.55-fold higher than those in the flask cultivation.
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