서강대학교

초록/요약

Cancer is a common and terrifying disease that ranks high in the cause of death worldwide. The earlier the discovery, the greater the likelihood of treatment. One commonly used diagnostic method is the ELISA assay, which measures the biomarker concentration. However, the ELISA method has a disadvantage that it is cumbersome and takes a long time. To overcome these advantages, an immunosensor chip was designed and it is different from other chips using a strip or an external pump for a fluid flow By using glass as a substrate, non-specific binding is minimized and capillary phenomenon eliminates the need for additional equipment. Aslo, by replacing the antibody with an aptamer, the shelf life of the chip was extended by increasing the stability for temperature. The PSA concentration, which is a biomarker of prostate cancer, was measured by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The peak height of the graph was also risen with increasing concentration in the range from 1 ng/mL to 10 ng/mL. In addition, to increase the sensitivity and accuracy of the chip, gold nanoparticles adhered to the surface of aptamer were applied instead of aptamer to reduce the limit of detection (LOD) to 0.1 ng/mL and to increase the difference in signal size according to the concentration. This study complements the disadvantages of existing chips and improves convenience and performance. If miniaturization of measuring instruments is made, it will bring about a breakthrough change in related fields by eliminating the restriction of diagnosis place.