서강대학교

초록/요약

Studying cell mechanical properties helps understanding cell’s dynamic behavior response to stress in tissue and circulation. Also, cell mechanical properties change during disease progress. In this Study, we measured mechanical properties of cellular membrane using micropipette aspiration. Micropipette aspiration is one of biomechanical assay which measure mechanical properties of cell or membrane by sucked membrane partially through micro-radius pipette. First, we studied effect of melittin to living cell’s mechanical properties. Melittin is one of antimicrobial peptides which are forming pore on membrane. When melittin formed pore, living cell showed more stiffness. These results mean that pore forming by melittin make membrane rigid. And, it is concluded that rigid pore and interaction between pore affect mechanical properties more than membrane thining effect. Second, we studied how extracellular matrix (ECM) affect to cell mechanical properties. ECM, including collagen, fibronectin and laminin, exist outside of living cell’s membrane with fiber form. In previous paper, that non-extensible collagen fiber make lysis tension increase in GUV model has been known. In this study, we investigated how very extensible fibronectin fiber change mechanical properties of membrane in GUV model. When GUV was coated fibronectin fiber, modulus became lower in low pressure. But, in higher pressure, modulus became increase compared with non-coated GUV which showed constant modulus. This results means that fibronectin influence to mechanical properties of cell in different way depending on stress.