Toxic effects of titanium dioxide nanoparticle on microbial activity and metabolic flux
- 발행기관 서강대학교 일반대학원
- 지도교수 이진원
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 석사
- 학과 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000045904
- 본문언어 영어
- 저작권 서강대학교의 논문은 저작권에 의해 보호받습니다
초록/요약
The purpose of this research is to estimate and quantify toxicity of titanium dioxide (TiO2) nanoparticle for microorganism. Nano size particle of TiO2 was more toxic than micro size a particle. Three species of microorganism, namely, Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae were tested to test antibacterial effect. E. coli showed the lowest survival ratio (36%) and S. cerevisiae was the most resistant to toxicity of TiO2 nanoparticles (71%). The antibacterial effect of TiO2 is also dependent on wavelength of ultraviolet ray. Survival ratio of E. coli was 40 % in 254 nm wavelength and 80% in 365 nm. To observe the effect of TiO2 on intracellular metabolite, metabolic flux analysis was performed in addition to measurement of in vivo glucose-6-phosphate. G6P concentration of cell exposed to TiO2 increased and glycolysis flux higher than control.
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