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Microbial Biogas Valorization for the Simultaneous Production of Ectoine and NaHCO3

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1. Introduction 11
1.1. Research background 11
1.2. Overview of this study 13
1.3. Salting-out process design 16
2. Material and Methods 20
2.1. Bacterial strain and medium 20
2.2. Flask cultivation 23
2.3. Fermenter cultivation 25
2.4. Salting-out process through evaporative crystallization 27
2.5. Analytical methods 28
2.6. Techno-economic analysis 29
3. Results and Discussion 31
3.1. Fermenter operation with increased initial inoculation OD 31
3.2. Effects of biogas impurities on cell growth and ectoine production 33
3.3. Effects of salt concentration on cell growth and ectoine production 36
3.4. Continuous fermenter cultivation using biogas 38
3.5. Salting-out process for NaHCO3 production 43
3.6. Production rate and energy distribution 50
3.7. Techno-economic analysis 53
3.8. Sensitivity analysis and carbon conversion analysis 57
4. Conclusion 60
5. Acknowledgement 62
6. References 63

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