서강대학교

목차

CONTENTS ⅰ
LIST OF FIGURES ⅳ
LIST OF ABBREVIATIONS ⅴ
PRIMER LIST ⅵ
초록 ⅸ
ABSTRACT ⅺ
INTRODUCTION 1
MATERIALS AND METHODS 4
Plant materials and growth conditions 4
Freezing assay 4
Electrolyte leakage assay 5
RNA extraction and cDNA synthesis 6
Quantitative RT-PCR 7
Semi-quantitative RT-PCR 7
Plasmid Construction and Agrobacterium Transformation 8
RNA-seq Analysis 8
RESULTS 10
Accession-specific differences in freezing tolerance are consistently revealed in both non-
acclimated and cold-acclimated conditions 10
Establishment of CBF-deficient genetic backgrounds for accession-specific cold-response analysis 13
Functional loss of CBF-dependent transcriptional activation in cbf123 deletion mutants 15
Basal CBF activity mitigates cellular freezing injury but does not alter whole-plant survival in non-acclimated conditions 18
Accession-specific factors modulate freezing tolerance beyond the CBF pathway in cold-acclimated
conditions 23
Distinct early cold-induced transcriptome reprogramming separates tolerant and sensitive accessions 27
A naturally occurring 5′UTR insertion is associated with reduced CBF1 induction in C24RDLUC 32
Removal of the C24 5′UTR insertion restores early cold-induced CBF1 expression levels 37
Identification of early cold-responsive genes that differentiate cbf123 deletion mutants 40
Validation of CBF-independent candidate genes contributing to accession-specific cold tolerance 45
DISCUSSION 49
REFERENCES 56