Proteolytic Regulation of PAX8 Through Dual Degradation Pathways
- 발행기관 서강대학교 일반대학원
- 지도교수 김종찬
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제URI http://www.dcollection.net/handler/sogang/000000082672
- UCI I804:11029-000000082672
- 본문언어 영어
- 저작권 논문은 저작권에 의해 보호받습니다.
목차
List of Figure ⅰ
초록 ⅱi
Abstract iv
Introduction 1
Materials and Methods 5
Antibodies and Reagents 5
Cell Lines 6
Plasmids 6
Immunoblotting 7
Site-Directed Mutagenesis 8
Immunoprecipitation and Pulldown Assays 9
Ubiquitination Assay 10
Deubiquitination Assay 10
Luciferase Reporter Assay 11
Cycloheximide Chase assay 12
Statistical Analysis 12
Results 14
PAX8 is regulated via proteasomal degradation pathway 14
PAX8 is also regulated via lysosomal degradation pathway 18
PAX8 protein regulation via both degradation pathways is ubiquitination dependent 21
Screening for PAX8-interacting deubiquitinases 25
Screening for deubiquitinases regulating PAX8 transcriptional activity 29
Confirmation of direct interaction between PAX8 and USP21 32
PAX8 and USP21 interact with each other via PRD, HD and USP domains, respectively 35
USP21 deubiquitinates PAX8 40
USP21 promotes PAX8 protein stability 44
USP21 prevents autophagy-lysosomal degradation of PAX8 46
BAY-805 fails to inhibit USP21 enzymatic activity 50
Discussion 55
References 64
