Immuno-Membrane Device for Exosome Capture and Dual-Mode Electrochemical and Colorimetric Detection of Exosomal Biomarkers
- 발행기관 서강대학교 일반대학원
- 지도교수 오병근
- 발행년도 2026
- 학위수여년월 2026. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000082524
- UCI I804:11029-000000082524
- 본문언어 영어
- 저작권 논문은 저작권에 의해 보호받습니다.
초록(요약문)
Exosomes are nanoscale extracellular vesicles (approximately 30–200 nm) that carry molecular information reflective of their cells of origin; however, reliable comparative analysis of exosomal biomarkers remains challenging due to variations in exosome number and the complexity of conventional isolation and normalization procedures. Here, we present a simple and practical dual-mode immuno-membrane platform that enables standardized comparative analysis of exosomal biomarkers within a single workflow. Exosomes were directly captured from cell culture media using a syringe-assisted bidirectional flow process without complex isolation steps, and their exosomal nature was confirmed by standard physicochemical and biochemical characterization. The number of captured exosomes was quantified electrochemically via CD63-mediated capture using electrochemical impedance spectroscopy, while the exosomal surface biomarker CD24 was evaluated through a colorimetric assay on the same membrane. Direct comparison of unnormalized colorimetric signals revealed strong dependence on exosome number. By extracting exosome numbers from ΔRct values and normalizing the colorimetric signals accordingly, variability caused by unequal exosome capture was minimized, enabling reliable per-exosome biomarker comparison. This normalized dual-mode strategy enabled clear discrimination between MCF-7- and MCF-10A-derived exosomes. Overall, this work introduces a utility-oriented analytical framework that combines minimal preprocessing, syringe-based operation, and electrochemical normalization, providing a practical approach for standardized exosome profiling and comparative biomarker analysis. Keywords: Exosome; Immuno-membrane; Direct exosome capture; Syringe-assisted flow; Exosome normalization
more목차
Abstract 1
1. Introduction 3
2. Materials and Method6
2.1 Materials 6
2.2 Preparation of Gold-Coated Immuno Membrane7
2.3 Cell Culture and Collection of Exosome Conditioned Media- 8
2.4 Exosome Capture on the Immuno-Membrane Using a Bidirectional Syringe-
8
2.5 Preparation of electrochemical sensing system for detection of exosome
surface protein9
2.6 Preparation of colorimetric sensing system for detection of exosome surface
protein-10
3. Results and Discussion-13
3.1 Fabrication process and comprehensive characterization of the Au-immuno-
membrane device. 13
3.2 Characterization of MCF-7-derived exosomes 17
3.3 Optimization of the Au-Immuno-Membrane Device for Efficient Exosome
Capture 19
3.4 Colorimetric Detection of CD24 on Captured Exosomes. 22
3.5 Electrochemical Detection of Exosomes of CD63 24
3.6 Application to MCF-10A-Derived Exosomes and Comparative Analysis-26
3.7 Normalized Dual-Mode Signal Analysis for Comparative Exosome Profiling
32
Conclusion 36
References 38
