Intratumoral delivery of Cxcl9 mRNA or Cd59 siRNA for enhanced immunotherapy
- 주제어 (키워드) Cancer immunotherapy , hot tumor , CXCL9 , CD59 , intratumoral injection , 암 면역치료 , 뜨거운 종양 , CXCL9 , CD59 , 종양 내 주사
- 발행기관 서강대학교 일반대학원
- 지도교수 이갑열
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000079780
- UCI I804:11029-000000079780
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
1. Introduction 1
2. Materials and Methods 4
3. Results 13
1) mRNA level and protein expression increased in B16 tumor cells after transfection with five mRNA targets 13
2) Intratumoral injection of Cxcl9 mRNA to B16 tumor reduced tumor growth and elevated CXCL9 secretion within tumor 18
3) Local delivery of Cxcl9 mRNA to B16 tumor improved anti-melanoma effects of T cells in vivo 21
4) Knockdown of Cd59 mRNA by Cd59 siRNA in B16 cell and tumor 29
5) Intratumoral injection of Cd59 siRNA to B16 tumor has no effect on tumor growth and mass, exhibiting no T cell antitumor immunity 32
6) Cd59 siRNA did not control the growth of tumors or exhibit anticancer effects of T cells in the MC38 colon adenocarcinoma model 41
4. Discussion 50
5. Reference 54
