Development of melittin modified lipid nanoparticles for intranasal administration vaccination
- 주제어 (키워드) Lipid Nanoparticle(LNP) , Surface Modification , Mucus Penetration
- 발행기관 서강대학교 일반대학원
- 지도교수 김현철
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000079606
- UCI I804:11029-000000079606
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
1. Introduction 1
1.1. mRNA lipid nanoparticle (LNP) 1
1.2. Vaccine delivery system 7
1.3. Hypothesis 13
2. Materials and Methods 16
2.1. Materials 16
2.2. Conjugation of Lipid-Melittin (DOPE-MEL) 17
2.3. Fabrication of melittin modified lipid nanoparticles (LNP-MEL) 18
2.4. In vitro cellular uptake efficiency of LNP-MEL 19
2.5. Mucus layer penetration efficiency of LNP-MEL 21
3. Results and Discussion 24
3.1. Characterization of LNP-MEL 24
3.2. Enhanced cellular uptake of LNP-MEL in a monolayer model 27
3.3. Enhanced endosomal escape efficiency of LNP-MEL in a cellular monolayer model 31
3.4. Enhanced cellular uptake of LNP-MEL on mucus layered cell model 35
4. Conclusion 40
5. References 42
