서강대학교

목차

Chapter 1. Extracellular Matrix and Cell Interactions: Mechanotransduction, Wound Healing, and Therapeutic Applications 1
Abstract 1
1.1 Introduction 2
1.2 Cellular Mechanotransduction 3
1.3 Regulation of ECM in Skin Wound Healing 5
1.4 Extracelluar Matrix-Based Therapeutic Applications 8
1.5 Conclusions 10
Chapter 2. Elevated Expression of α5β1 Integrin Alters Integrin Clustering and Extracellular Matrix-Cellular Interactions in Human Dermal Fibroblast 12
Abstract 12
2.1 Introduction 13
2.2 Materials and Methods 15
2.3 Results and Discussion 19
2.3.1 Overexpression of α5β1 Integrin in Human Dermal Fibroblast 19
2.3.2 Overexpression of α5β1 Integrin Alters the Dynamics of Integrin Clustering 21
2.3.3 Overexpression of α5β1 Integrin Increases ECM-Integrin and Cell-Cell Interactions 24
2.4 Conclusions 28
Chapter 3. Scalable Extracellular Matrix Spray Coating for Enhanced Cellular Adhesion and Accelerated Wound Healing 29
Abstract 29
3.1 Introduction 30
3.2 Materials and Methods 32
3.3 Results and Discussion 39
3.3.1 Spray Induces the Unfolding of Fibronectin Structure 39
3.3.2 ECM Spray Enhances Cellular Adhesion on Different Substrates 43
3.3.3 Effects of Multi-ECM Spray on ECM Directionality and Cellular Activities 46
3.3.4 Sequential ECM Spray Accelerates Wound Healing 50
3.4 Conclusions 54
Chapter 4. Topical Nanoliposomal Collagen Delivery for Targeted Fibril Formation by Electrical Stimulation 56
Abstract 56
4.1 Introduction 57
4.2 Materials and Methods 60
4.3 Results and Discussion 66
4.3.1 Lip-Cols Characterization 66
4.3.2 Collagen Fibrils Formation from Lip-Cols Stimulated by Electric Fields 68
4.3.3 Self-assembly of Collagen Monomers under Electric Fields 71
4.3.4 Collagen pH Gradient and Structural Conformation Changes under Electric Fields 74
4.3.5 Lip-Cols Influence Collagen Fibril Orientation on Human Dermal Fibroblasts 76
4.3.6 Lip-Cols and EF Enhance the Permeability of Collagen Monomers in Skin Layers 80
4.4 Conclusions 86
References 87