Development of Microbubble conjugated Nanoparticle Loaded with CRISPR Plasmid for Targeted Delivery and Sonodynamic and Photodynamic Therapy
- 주제어 (키워드) CRISPR/Cas9 Plasmids , Nanoparticles , Microbubbles , Ultrasonication , Photodynamic Therapy , 크리스퍼/카스9 플라스미드 , 나노입자 , 마이크로버블 , 초음파 , 광역학 치료
- 발행기관 서강대학교 일반대학원
- 지도교수 김현철
- 발행년도 2025
- 학위수여년월 2025. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000079343
- UCI I804:11029-000000079343
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
1. Introduction 1
1.1. Gene editing technology development 1
1.2. CRISPR/Cas9 delivery system 7
1.3. Ultrasonic-Responsive delivery system 10
1.4. Photodynamic Therapy 15
2. Materials and Methods 18
2.1. Materials 18
2.2. Cell culture 20
2.3. Fabrication of DOTAP NPs and DOTAP-PEG NPs, Microbubbles and Chlorin e6 Microbubbles and DOTAP-PEG NPs Microbubbles complex 20
2.4. Characterization of DOTAP NPs and DOTAP-PEG NPs, Microbubbles and Chlorin e6 Microbubbles and DOTAP-PEG NPs Microbubbles complex 21
2.5. In vitro Cell cytotoxicity analysis 22
2.6. Hemolysis assay 22
2.7. Serum stability analysis 23
2.8. In vitro Knock-out efficiency analysis 23
2.9. Measuring ROS generation inside the cells 24
2.10. Western blot 25
3. Results and Discussion 26
3.1. Characterization of DOTAP NPs and DOTAP-PEG NPs, Microbubbles and DOTAP-PEG NPs Microbubbles complex 26
3.2. GFP knock-out efficiency using GFP knock-out CRISPR plasmid DNA 30
3.3 Confirmation chlorin e6 loaded Microbubbles 33
3.4 Photodynamic Therapy ability of Chlorin e6 loaded microbubbles 36
3.5. Preparation for in vivo experiments 39
3.6 Real-Time Therapeutic B-mode Imaging Using High Intensity Focused Ultrasound 42
4. Conclusion 45
5. References 47
