Development of Fabry disease kidney organoid-on-a-chip for gradient drug screening
- 주제어 (키워드) Kidney organoids , Fabry disease , Microfluidic gradient chip , Enzyme replacement therapy (ERT) , Drug screening
- 발행기관 서강대학교 일반대학원
- 지도교수 정봉근
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 융합의생명공학과협동과정
- 실제 URI http://www.dcollection.net/handler/sogang/000000079115
- UCI I804:11029-000000079115
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
I. Introduction 6
II. Methods and Materials 9
i. Design and fabrication of microfluidic gradient chip 9
ii. Computational fluid dynamics (CFD) simulation 9
iii. Human induced pluripotent stem cells (hiPSCs) culture and kidney organoids differentiation 10
iv. Generation of Fabry disease kidney organoids 11
v. Preparation on microfluidic chip to culture kidney organoids 14
vi. Immunofluorescence analysis 14
vii. Quantitative RT-PCR 15
viii. Mitochondrial staining and reactive oxygen species (ROS) detection 16
ix. Statistical analysis 17
III. Results and discussions 17
i. Generation of Fabry disease kidney organoids in a microfluidic chip 17
ii. Enhancement of differentiation and maturation of normal kidney organoids in fluidic culture conditions 22
iii. The continuous flow of medium enhances the differentiation and maturation of Fabry disease kidney organoids 26
iv. ERT reduced Gb3 accumulation and attenuated structural and transcriptional changes in Fabry disease kidney organoids 30
v. ERT attenuated oxidative stress and mitochondrial dysfunction in Fabry disease kidney organoids 34
IV. Conclusion 37
V. References 38
