Development of a melittin based nanomedicine modified with phospholipid for anti-inflammatory treatment
- 주제어 (키워드) Nanomedicine , Cleavable lipid-melittin , Redox-responsive release
- 발행기관 서강대학교 일반대학원
- 지도교수 김현철
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000078984
- UCI I804:11029-000000078984
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
1. Introduction 1
1.1. Background 1
1.2. Pathogenesis of RA 2
1.3. Symptoms and treatment of RA 5
1.4. RA treatment with nanoparticles 7
1.5. Melittin 8
1.6. Necessity of nanotechnology 10
2. Materials and Methods 12
2.1. Materials 12
2.2. Preparation of PE-s-s-Cmel 13
2.3. Characterization of PE-s-s-Cmel 13
2.4. Preparation of liposomes (DM12 and PE liposome) 14
2.5. Characterization of liposomes (DM12 and PE liposome) 15
2.6. Cell culture 15
2.7. In Vitro flow cytometry and endosomal escape imaging 16
2.8. In Vitro gene expression 17
2.9. In Vitro cytotoxicity assay and hemolysis assay 18
2.10. In Vitro drug release study 19
2.11. Statistical analysis 20
3. Results and Discussion 22
3.1. Characterization of PE-s-s-Cmel and DM12 22
3.2. Cytotoxicity assay 28
3.3. Cellular uptake of DM12 32
3.4. Inflammatory cytokine PCR analysis of DM12 36
3.5. Characteristics of DM12 with MTX 39
4. Conclusion 42
5. References 44
