The impact of IL-18 signaling on Tumor-infiltrating Regulatory T cells
- 주제어 (키워드) Tumor-infiltrating Treg cells; 종양 침윤 조절 T 세포 , IL18R1
- 발행기관 서강대학교 일반대학원
- 지도교수 이갑열
- 발행년도 2024
- 학위수여년월 2024. 8
- 학위명 석사
- 학과 및 전공 일반대학원 생명과학과
- 실제 URI http://www.dcollection.net/handler/sogang/000000078936
- UCI I804:11029-000000078936
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
Ⅰ. Introduction 1
Ⅱ. Materials and Methods 4
1. Mice 4
2. Cell line 4
3. In vitro differentiation of nave CD4+ T cells 4
4. Isolation of tdLN Treg cells 5
5. RNA isolation and quantitative reverse transcription polymerase chain reaction (qRT-PCR) 6
6. RNA-sequencing (RNA-seq) data analysis 8
7. Tumor model 8
8. Suppression assay 8
9. Flow cytometry 9
10. Adoptive T cell transfer 10
11. Statistical analysis 10
Ⅲ. Results 11
1. IL18R1 deficient mice show delayed tumor growth and IL18R1 expression indicates effector Treg cell population 11
2. IL18R1 deficiency enhances Treg cell differentiation 14
3. IL-18 signaling impairs Foxp3 expression and Treg cell proliferation 18
4. IL-18 signaling does not affect the suppressive function of Treg cells 23
5. IL18R1 deficiency does not alter Treg cell infiltration or marker expression in the tumor microenvironment 26
6. Transfer of Il18r1 KO Treg cells does not affect tumor growth in immunodeficient mice 33
Ⅳ. Discussion 37
Ⅴ. References 42
