Effect of specific growth rate on polyhydroxybutyrate production in high cell density culture of recombinant Escherichia coli
- 주제어 (키워드) Biodegradable polymers , polyhydroxybutyrate , recombinant strain , fed-batch high cell density cultivation , feedback control , specific growth rate
- 발행기관 서강대학교 일반대학원
- 지도교수 나정걸
- 발행년도 2024
- 학위수여년월 2024. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000077075
- UCI I804:11029-000000077075
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권 보호를 받습니다.
목차
1. Introduction 1
2. Materials and Methods 5
2.1. Recombinant strain and culture conditions 5
2.2. Medium preparation for cell growth 5
2.2.1. Seed culture (flask-scale) 6
2.2.2. Main culture (bioreactor-scale) 7
2.3. Setup of lab-scale bioreactor 8
2.4. Fed-batch culture system 9
2.5. Analytical methods 10
3. Results and Discussion 12
3.1. Correlation between optical density and dried cell mass 12
3.2. Batch culture test of recombinant strain 13
3.3. Cell growth of recombinant E. coli in different μ control 15
3.3.1. Inaccurate feeding algorithm and composition for early stage of feeding 19
3.3.2. Accumulation of toxic chemical during fed-batch culture 21
3.4. Comparison between cell growth and PHB synthesis in different μ 22
3.4.1. Trend analysis of effecting factor in PHB analysis 26
3.4.2. Adjusting C-N ratio in feeding solution for enhanced production of PHB 27
4. Conclusion 30
5. References 33
