서강대학교

초록 (요약문)

Parkinson’s disease (PD) is one of the most common neurodegenerative disorders caused by loss of dopaminergic neurons in the area of the brain called substantia nigra. Since no significant symptoms are found in PD patients until more than 60% of dopaminergic neurons in midbrain have been lost, sensitive dopamine (DA) detection is important for early detection of PD. In this research, a mesoporous nanopatterned 3D concave electrode was developed to evaluate drug effects of PD using patient-derived midbrain organoids who have found a gene mutation in LRRK2 and PRKN, respectively. Mesoporous nanoscale dots were patterned on Au-sputter coated film by laser interference lithography (LIL) technique for fabrication of mesoporous nanopatterned 3D concave electrode. The mesoporous structure and nanodots increase the surface area of the electrode, enabling to detect DA level with high sensitivity. After drug treatment, the electrochemical signal recovery of PD organoids using the fabricated 3D concave electrode was shown. The proposed 3D concave electrode is expected to be utilized in a platform technology broadly applicable to disease detection and drug screening of other neurodegenerative diseases.