Pumpless three-dimensional photo paper-based microfluidic analytical device for automatic detection of thioredoxin-1 using enzyme-linked immunosorbent assay
- 주제(키워드) 도움말 Photo paper , Microfluidics , Colorimetric , ELISA , Thioredoxin-1 , Point-of-care
- 발행기관 SPRINGER HEIDELBERG
- 발행년도 2021
- 총서유형 Journal
- 본문언어 영어
초록/요약 도움말
Microfluidic-based biosensors have been developed for their precise automatic reaction control. However, these biosensors require external devices that are difficult to transport and use. To overcome this disadvantage, our group made an easy-to-use, cheap, and light pumpless three-dimensional photo paper-based microfluidic analytical device (3D-mu PAD; weight: 1.5 g). Unlike conventional paper-based microfluidic analytical devices, the 3D-mu PAD can be used to control fluid flow in a 3D manner, thus allowing sophisticated multi-step reaction control. This device can control fluid flow speed and direction accurately using only the capillary-driven flow without an external device like a pump. The flow speed is controlled by the width of the microfluidic channel and its surface property. In addition, fluid speed control and 3D-bridge structure enable the control of fluid flow direction. Using these methods, multi-step enzyme-linked immunosorbent assay (ELISA) can be done automatically in sequence by injecting solutions (sample, washing, and enzyme's substrate) at the same time in the 3D-mu PAD. All the steps can be performed in 14 min, and data can be analyzed immediately. To test this device, thioredoxin-1 (Trx-1), a biomarker of breast cancer, is used as the target. In the 3D-mu PAD, it can detect 0-200 ng/mL of Trx-1, and the prepared 3D-mu PAD Trx-1 sensor displays excellent selectivity. Moreover, by analyzing the concentration of Trx-1 in real patients and healthy individuals' blood serum samples using the 3D-mu PAD, and comparing results to ELISA, it can be confirmed that the 3D-mu PAD is a good tool for cancer diagnosis.
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