서강대학교

초록 (요약문)

Acid-resistant coatings are used in various fields with diverse materials. However, most of them are limited to the industrial field, and studies on the biological application of acid-resistant coatings are insufficient. This study focuses on the extraordinary acid resistance of keratin protein and confirms whether acid resistance can be used as an actual coating material. It also verifies whether this acid resistance can be further enhanced by treating formic acid, which is known to reinforce the β-structure of keratin. Keratin films were made by using deionized water (KD film) and formic acid (KF film) as solvents. Gelatin-DIW films were made with the same mechanism. The film was formed by dropping solutions onto a PTFE sheet, drying in a drying oven at 60°C, and cross-linking in a sealed container with glutaraldehyde vapor. In addition, detailed conditions were determined through an optimization experiment. The films made were compared in appearance through a visual and Low-voltage scanning electron microscope. In an experiment comparing acid resistance, the films were finely pulverized and then immersed in an aqueous hydrochloric acid solution adjusted to pH 1 and 0 with deionized water as a control group. After then, each spectrum was analyzed with 2D Fourier-transform infrared spectroscopy. The major peak Amide I was analyzed in detail using the Attracted total reflection accessory. In the results, KF film showed a smoother and less curved surface shape than KD film in appearance. Comparing the changes in films after acidic treatments with IR spectral analysis, both keratin films showed significantly smaller spectral changes than gelatin films, especially in KF films. In the detailed analysis of the Amide I peak, KF film also showed a higher β-structural content than KD film.