서강대학교

초록/요약

The three dimensional (3D) neural cell models such as neurospheroid and brain organoid have been widely studied for demonstration of degenerative brain disease model to be applied in drug screening. To investigate the effects of neurotransmitters or neurodegenerative drugs to 3D neural cell models in biocompatible manner, non-destructive monitoring method for 3D neural cell models is essentially required. To achieve this goal, in this research, we proposed the nanobiohybrids, which are composed of protein, DNA, carboxyl-modified molybdenum disulfide nanoparticles (cMoS2), Arg-Gly-Asp tripeptide (RGD) and cell penetrating peptide (CPP), to demonstrate the non-destructive monitoring tool for drug screening in 3D neural cell model. Proposed nanobiohybrids can be half-penetrated into 3D neurospheroid, control and measure the intracellular charge states signals. Moreover, by combining the microelectrode array (MEA) and the nanobiohybrids, the selective charge control and non-destructive signal monitoring of 3D neurospheroid was demonstrated to monitor the excitatory effect of glutamate on 3D neurospheroid. This newly developed system can provide the novel method to monitor the effects of various neurotransmitter and drugs for degenerative brain disease in non-destructive manner.