서강대학교

초록/요약

Biofuel production by syngas fermentation has been studied and reported. The process of production of ethanol using carbon monoxide as feedstock has both marketability and commercial viability [1]. However, for successful ethanol production, we need to overcome the limits of high-concentration cell culture [2, 11, 16]. In order to pass this limitation, experiments about cell growth were performed on Clostridium autoethanogenum with various carbon sources and culture conditions. Research conducted in serum vials showed that Clostridium autoethanogenum can grow much faster in heterotrophic growth using fructose as a carbon source. Fed on fructose, C. autoethanogenum’s cell density reaches 16(±0.89) of 〖OD〗_600 on fill and draw operation. And after heterotrophic growth, C. autoethanogenum of high concentration was able to adapt well to lithotrophic growth using syngas as a carbon source. And this high cell density cultivation technique was also performed in CSTR bioreactor using hollow fiber filtration module. In 248 hours of heterotrophic growth, cell density reaches 18.942 of OD600. Then the culture medium was replaced with a fresh new medium by hollow fiber module to eliminate fructose. The cell density of C. autoethanogenum decreases from 18.942 to 3.641 over 26 days, and the amount of produced ethanol in the condition was 25.1 g/L.