Chemostat culture of Methylomonas sp. DH-1 to investigate methane assimilation characteristics
- 주제(키워드) Methylomonas sp. DH-1 , methane , chemostat , methanol , cell activity
- 발행기관 서강대학교 일반대학원
- 지도교수 이진원
- 발행년도 2019
- 학위수여년월 2019. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000063822
- UCI I804:11029-000000063822
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권보호를 받습니다.
초록/요약
Methylomonas sp. DH-1 is a type I methanotroph that uses methane as a carbon source. This bacterium is one of the brightest and most promising strains near future in that it can use methane gas, one of the harmful green-house gases that cause global warming. In this study, Methylomonas sp. DH-1 was cultured in 5 L fermenter with chemostat culture, one of the continuous cultures. Chemostat culture is a device that constantly regulates productivity of microorganisms with maximum cell concentration under each condition. By comparing the maximum concentration and productivity of cell under various conditions, the difference in cell activity was studied. Here, the effect of agitation speed and dilution rate on cell productivity and concentration was confirmed in chemostat culture. In addition, the characteristics of methane assimilation of cell depending on the culture characteristics were investigated. In the chemostat culture, methanol bioconversion from methane were carried out using cells harvested under each condition. The difference in methanol production by cell activity was confirmed. These results show that culture characteristics is important to improve the productivity of compounds synthesized using microorganisms.
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