서강대학교

초록/요약

Cell membranes have lipid bilayer and protein, cytoskeleton, extracellular matrix. The extracellular matrix (ECM) has important roles in regulating the development, function and homeostasis of all eukaryotic cells. Specifically, fibronectin is known to exhibit very elongated properties due to folded domains. Laminin, on the other hand, is a basal membrane protein that tends to be coiled into three short polypeptide bonds. However, despite this physiological significance, the mechanism of attachment of ECM and fiber structural characteristics are still unclear. Essentially, binding of ECM molecules to integrin induces ECM unfolding, thereby spontaneously promoting interactions and fibril assembly. With simplified model of cell, ECM binding GUV, we can get insight of each component role in cell. By using this cell, we can measure mechanical properties and we can see biological phenomenon role. For fibrillogenesis of ECM such as laminin and fibronectin outside of vesicle, vesicles were made with PS lipid with negative charge ahead. ECM fiber can be coated on GUV by negative charge. GUV were incubated with ECM dimer at 37℃ during 12hrs. As a result, ECM coated vesicle was obtained. This study examined how the extracellular matrix (ECM) deposite on charged lipids. Confocal image include osmotic pressure and micropipette aspiration were used to measure the mechanical properties of cell membranes for 3 dimensional surfaces. And AFM was used to analyze extracellular matrix’s molecular structure for 2 dimensional surfaces. When GUV is coated with fibronectin fiber, it exhibits low modulus and occurs budding phenomenon, laminin fiber occur cracking and shrinking when we put hypertonic stress because they has high area expansion modulus.