서강대학교

초록/요약

Multiple drug resistance is a critical barrier to successful chemotherapy. Among the investigated mechanisms of drug resistance, the P-glycoprotein mediated anticancer drug resistance has received the most attention. In this study, we apply photodynamic therapy with chemotherapy using human serum albumin nanoparticles with doxorubicin and microbubbles containing Chlorin e6 (DOX-NPs/Ce6-MBs) to overcome the multi-drug resistance and maximize the therapeutic effect. Microbubbles are used not only as a carrier of Chlorin e6, photosensitizer, but also as the functional structures for increasing the permeability of cell membranes with ultrasound irradiation. Moreover, reactive oxygen species generated by photodynamic therapy has been shown to have functional destruction effects on drug efflux pumps. The therapeutic effects of DOX-NPs/Ce6-MBs have confirmed in MCF-7/ADR cells expressing the P-glycoprotein. As a result, DOX-NPs/Ce6-MBs not only increases the efficiency of drug delivery and chemotherapy, but also effectively overcomes drug resistance by interfering with the drug efflux pumps, which is the main cause of the drug resistance, through combined photodynamic therapy. Consequently, we suggest that DOX-NPs/Ce6-MBs with ultrasound and laser can be an efficient platform for cancer therapy by overcoming multidrug resistance.