Bioconversion of 2,3-butanediol to mevalonate using recombinant Pseudomonas putida
- 주제(키워드) Pseudomonas putida , 2 , 3-Butanediol (2 , 3-BDO) , mevalonate , terpenoids , recombinant strain
- 발행기관 서강대학교 일반대학원
- 지도교수 오병근
- 발행년도 2018
- 학위수여년월 2018. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000062775
- 본문언어 한국어
- 저작권 서강대학교 논문은 저작권보호를 받습니다.
초록/요약
Studies on the biological production of 2,3-Butanediol (2,3-BDO), a C4 platform chemical, are active, but the cost of separating and purifying the produced 2,3-BDO is substantial. In this study, 2,3-BDO is converted into hydrophobic terpenoids using recombinant pseudomonas putida. The production of mevalonate when 2,3-BDO was used as a substrate was about 8 times higher than When glucose and glycerol was used as a substrate under the same conditions. The lower the aeration, the higher the yield of mevalonate in the same culture condition except the aeration. The maximum mevalonate production on the shaking flask scale was about 2.21 g/L in this study, considering the effects of aeration and substrate.
more