Crosslinking Effects on crystallization of lysozyme in Crosslinked Hydrogel matrices
- 발행기관 서강대학교 대학원
- 지도교수 신관우
- 발행년도 2016
- 학위수여년월 2016. 2
- 학위명 석사
- 학과 및 전공 일반대학원 화학과
- 실제URI http://www.dcollection.net/handler/sogang/000000059035
- 본문언어 영어
- 저작권 서강대학교 논문은 저작권보호를 받습니다.
초록/요약
The real cell has very small volume surrounded by the membrane and extremely crowded structures containing the various kinds of biological macromolecules. In these environments, biochemical reaction is different from the general environment. However, it is not clear that these crowed and confined environments influence the reaction whether faster or slower. In this study, to confirm the effect of biochemical reaction in the intercellular environment, we mimic the cell-like environment. Poly (ethylene glycol) diarylate (PEGDA) polymers are used to make the crowed environment. Then, to make the confined environment, we make the PEGDA cross-linked hydrogels that have internal pores by irradiating the UV. Hen egg white lysozyme (HEWL) is used the crystallized reactant to confirm the influence on the reaction in each environment.
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