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3차원 광결정 구조의 광 가이드 효과를 통한 단백질 검출 성능 향상에 관한 연구 : Study on improvement of protein detection using light guidance by 3D photonic crystals

초록/요약 도움말

Photonic crystal (PC) based biosensors have been attracted more attention due to their advantages such as sensitivity, real time monitoring and low-cost realization. PC based sensors have been applied to measure various environmental changes such as pH, temperature and glucose concentration. In this study, we characterize the intensity enhancement of quantum dots (Qdot) whose emission is guided by 3D photonic crystal (PC) structures for protein detection. We make that the emitted light from the PC based sensor recovered, when the chemical antibody (aptamer) conjugated with guard DNA (g-DNA) labeled with a quencher (Black FQ), detects our target protein (thrombin). The quencher has broad light absorbance property ranging from 500 to 700 nm (its maximum absorbance is 531 nm). It induces the quenching effect of fluorescence intensity in the Qdot-aptamer. In the presence of the target protein (thrombin), the Qdot-aptamer complex prefers to form the thrombin-aptamer complex: this results in the release of Black FQ-g-DNA and the quenched light intensity recovers the original high intensity with Qdot. The intensity recovery varies quantitatively according to the level of the thrombin concentration. Our scheme shows much higher detection sensitivity than the general fluorescent detection mechanism, which is functionalized on the flat surfaces because of the light guiding effect from 3D photonic crystal structures. In addition, the end user can detect the target directly and easily without any sample preparation routinely required for a ELISA kit.

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