초록/요약

Analysis and manipulation of neural stem cells are important for the neural engineering, especially for the field of regenerative medicine. In this dissertation, a new cutting-edge technology was developed to manipulate single neural stem cell, as well as to detect the differentiation of neural stem cell by analyzing its extracellular and intracellular state using graphene-based functional nanomaterials. Polymer-insulated nanoprobe was fabricated to monitor cell penetration and to deliver cargo into neural stem cell which was controlled by electrical self-assembly and release technique. GO-encapsulated gold nanoparticles conjugated with cell-penetrating peptide (GGCP particles) was then applied to identify the differences of intracellular state of undifferentiated/differentiated neural stem cell, which was superior to normal gold nanoparticles and CPP-non conjugated GO-encapsulated nanoparticles. Neural stem cells were further attached on graphene oxide (GO)-encapsulated gold nanostructures to analyze its differentiation potential based on spectroelectrochemical technique. The GO-encapsulated gold nanostructure was found to be excellent to detect the stem cell differentiation by analyzing its unsaturated/saturated state of cell membrane and its extracellular environment. The technique proposed in this research can be applied to the various kinds of stem cell-based researches including drug-screening, tissue engineering and regenerative medicine. Even more remarkably, this technique can potentially be applied to other types of stem cells (embryonic, mesenchymal and hematopoietic stem cells) and/or progenitor cells which are undergoing differentiation or are maintained in an undifferentiated state, because the unsaturated state of the cell membrane is a common characteristic of undifferentiated stem cells. Hence, the fabricated 3D ITO-60NP-GO structures can be used for various kinds of stem cell-based research and therapy and also as a rapid, sensitive and facile detection tool for determining both the undifferentiated and differentiated state of stem cells, leading to advancements in the practical use of stem cells as a new regeneration medicine.