A Rapid Detection Using Quantum Dots-Encapsulated Magnetic Bead
- 발행기관 서강대학교 일반대학원
- 지도교수 박정열
- 발행년도 2012
- 학위수여년월 2012. 2
- 학위명 석사
- 학과 및 전공 일반대학원 바이오융합기술협동과정
- 실제URI http://www.dcollection.net/handler/sogang/000000047340
- 본문언어 영어
- 저작권 서강대학교 논문은 저작원 보호를 받습니다.
초록/요약
This thesis proposes the development of an aptamer based assay employing the quantum dots (QDs)-encapsulated magnetic bead. Traditional assay usually involves multiple washing steps of significant time and labor cost. In order to improve these problems, I proposed a single-step assay process that can be integrated in microfluidic systems. Quenchers and specific aptamers were integrated on the QDs-encapsulated magnetic bead for single-step detection of protein. Real-time detection was confirmed by observing that thrombin binding reaction was completed within 10min after thrombin addition. The quantitative target protein was analyzed by the change of fluorescent intensity according to the concentration of protein. My new approach is expected to enable the rapid and specific protein detection with reduced costs and minimized materials consumption.
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