Converting sugars extracted from marine algae pretreatment to bio-ethanol by various ethanolic E.coli strains
- 주제(키워드) bioethanol , pretreatment , marine algae
- 발행기관 서강대학교 일반대학원
- 지도교수 이진원
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 석사
- 학과 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000045854
- 본문언어 영어
- 저작권 서강대학교의 논문은 저작권에 의해 보호받습니다
초록/요약
Marine algae, which take about 80% of the world’s living organisms, contain lots of energy sources, such as sugars and lipids. Therefore, possibility of utilizing structural carbohydrate from marine algae for bioethanol has been studied. In order to obtain mono sugars, Undaria pinnatifida, Chlorella vulgaris, and Chlamydomonas reinhardtii were used for the saccharification experiments. The pretreatment was carried out in dilute acid hydrolysis and enzymatic treatment. To find optimal condition, experiments were performed at several temperatures, acid concentrations, pH conditions and different period of time. To test bioethanol production, several ethanolic E. coli W3110 strains, which were developed previously, were used. The maximal yield of bioethanol was achieved with pretreated Chlorella vulgaris and E. coli SJL2526, derived from a wild type E. coli W3110, including adhB, pdc, galP and glk genes as 0.4 g-ethanol/g-biomass
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