Over-expressing sugar up-take genes in ethanolic Escherichia coli for effective conversion of Chlorella pre-treatment sugar into bio-ethanol
유전자 조작에 의한 효과적인 에탄올 생산 Escherichia coli 균주 개발 및 클로렐라 전처리물로의 에탄올 발효
- 주제(키워드) bio-ethanol
- 발행기관 서강대학교 화공생명공학대학원
- 지도교수 이진원
- 발행년도 2010
- 학위수여년월 2010. 2
- 학위명 석사
- 학과 일반대학원 화공생명공학과
- 실제URI http://www.dcollection.net/handler/sogang/000000045778
- 본문언어 영어
- 저작권 서강대학교의 논문은 저작권에 의해 보호받습니다
초록/요약
There are two main goals in this research. The first goal of this research is to develop an E. coli strain that is efficient in various sugars up take. And the second goal is to develop an E. coli strain which has an improved production yield of bio-ethanol. Both of these goals were achieved by developing a various ethanolic E. coli strains by gene mutation. Also the mutated ethanolic E. coli strains were 2L batch fermented with glucose and Chlorella pre-treatment sugar as the main carbon source, respectively. As a result, the maximal yield of bio-ethanol in Chlorella pre-treatment sugar batch fermentation was achieved in the 0.6% Chlorella pre-treatment sugar batch fermentation by pSJL25(: a pstv28 vector harboring pdc and adhb genes), and pSJL26(: a ptrc99a harboring galp and glk genes) inserted E. coli by a yield of 1.67g/L.
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