Cellular uptakes of colloidal nanoparticles functionalized by cell penetrating peptides
- 발행기관 서강대학교 대학원
- 지도교수 이원구
- 발행년도 2008
- 학위수여년월 2008. 8
- 학위명 석사
- 학과 및 전공 바이오융합기술협동과정
- 식별자(기타) 000000108436
- 본문언어 영어
목차
Cell-penetrating peptides (CPPs) are short peptides of less than 30 amino acids that are able to penetrate cell membranes and translocate different cargoes into cells. The only common feature of these peptides appears to be that they are amphipathic and net positively charged. Cargoes that are successfully internalized by CPP range from small molecules to proteins and supramolecular particles. The mechanism of cell translocation is not known but it is apparently receptor and energy independent although, in certain cases, translocation can be partially mediated by endocytosis. CPPs are novel vehicles for the translocation of cargo into cells, whose properties make them potential drug delivery agents, of interest for future use.
The functionalization of micro-, nano colloidal particles for novel drug delivery systems becomes an important area of research, since the uptake of drugs to a target cell is strongly influenced by the size, shape and chemical composition. It has been known that protein-transduction domains, known as cell penetrating peptides (CPP), can transverse cell membranes efficiently, thus translocate the attached cargo into the cytosol of the cell. In this thesis, I functionalized various sized CPP-coated silica particles (200 - 800 nm), and monitored the trans-membrane process into living mammalian cells using time lapse confocal microscopy and flow cytometry. I will discuss the cellular uptake efficiencies, as a function of surface charges and cargo size.
